Time-resolved investigation of nanometric cell membrane patches with a mid-infrared laser microscope
نویسندگان
چکیده
The proton pump Bacteriorhodopsin (BR) undergoes repeated photocycles including reversible conformational changes upon visible light illumination. Exploiting the sensitivity of infrared (IR) spectra to conformation, we have determined reaction kinetic parameters conductive intermediate M for wild-type protein and its slow mutant D96N during photocycle. Time-resolved IR micro-spectroscopy using an in-house developed confocal laser microscope operating in mid-IR is employed record absorption 10 −4 at wavelengths λ 1 = 6.08 μm 2 6.35 μm, assigned backbone retinal structural modifications, respectively. Protein samples were embedded dried lipid bilayers deposited on ultraflat gold supports enhance surface field. signals analyzed according a simplified photocycle model with only two dominant states: dark-adapted state BR* M. We obtained excitation relaxation times from exponential fits change time traces. Our results constitute first step towards future plasmonic-assisted nanoscale time-resolved spectrometers characterization bioelectronic light-harvesting nanodevices based BR.
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ژورنال
عنوان ژورنال: Frontiers in photonics
سال: 2023
ISSN: ['2673-6853']
DOI: https://doi.org/10.3389/fphot.2023.1175033